High-resolution allele frequencies for NGS based HLA-A, B, C, DQB1 and DRB1 typing of 23,595 bone marrow donors recruited for the Polish central potential unrelated bone marrow donor registry

Klaudia Nestorowicz; Anna Bogacz; Alicja Bukowska; Mateusz Chraplak; Jarosław Czerwiński; Michał Góralski; Mariusz Gronkowski; Karol Jopek; Łukasz Kniżewski; Michał Kolasiński; Marek L Kowalski; Jacek Nowak; Mateusz Sowiński; Sylwia Wróblewska-Kabba; Bogusław Tymoniuk; Małgorzata Dudkiewicz

doi:10.1016/j.humimm.2019.12.005

High-resolution allele frequencies for NGS based HLA-A, B, C, DQB1 and DRB1 typing of 23,595 bone marrow donors recruited for the Polish central potential unrelated bone marrow donor registry

Hum Immunol. 2020 Feb-Mar;81(2-3):49-51. doi: 10.1016/j.humimm.2019.12.005. Epub 2020 Jan 2.

Authors

Klaudia Nestorowicz¹, Anna Bogacz², Alicja Bukowska², Mateusz Chraplak², Jarosław Czerwiński³, Michał Góralski², Mariusz Gronkowski², Karol Jopek², Łukasz Kniżewski⁴, Michał Kolasiński², Marek L Kowalski⁵, Jacek Nowak⁶, Mateusz Sowiński², Sylwia Wróblewska-Kabba², Bogusław Tymoniuk⁵, Małgorzata Dudkiewicz⁷

Affiliations

¹ Central Unrelated Potential Bone Marrow Donor And Cord Blood Registry Poltransplant, 87 Al. Jerozolimskie Str, 02-005 Warszawa, Poland.
² Regional Centre of Blood Donation and Blood Treatment in Poznan, 44 Marcelińska Str., 60-354 Poznań, Poland.
³ Central Unrelated Potential Bone Marrow Donor And Cord Blood Registry Poltransplant, 87 Al. Jerozolimskie Str, 02-005 Warszawa, Poland; Department of Emergency Medicine, Medical University of Warsaw, 61 Żwirki i Wigury Str., 02-091 Warsaw, Poland.
⁴ Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 5a Pawinskiego Str., 02-106 Warsaw, Poland.
⁵ Department of Immunology, Rheumatology and Allergy, Medical University of Lodz, 251 Pomorska Str., Lodz, Poland.
⁶ Department of Immunogenetics, Institute of Hematology and Transfusion Medicine, 14 Indira Gandhi Street, 02-776 Warsaw, Poland.
⁷ Central Unrelated Potential Bone Marrow Donor And Cord Blood Registry Poltransplant, 87 Al. Jerozolimskie Str, 02-005 Warszawa, Poland; Department of Biochemistry and Microbiology, Warsaw University of Life Sciences, 159 Nowoursynowska Str., 02-776 Warsaw, Poland. Electronic address: m.dudkiewicz@poltransplant.pl.

PMID: 31902540
DOI: 10.1016/j.humimm.2019.12.005

Abstract

Next-generation sequencing (NGS)-based typings of HLA-A, B, C, DQB1 and DRB1 loci were performed from 2018 to 2019 in 23 595 newly recruited or re-typed adult potential bone marrow donors registered in Poltransplant Registry to characterize allele and haplotype frequencies of HLA system for loci important for hematopoietic stem cell transplantation. The donors were recruited for registry and not for any other purpose including controls in a disease association study. The population sample was collected in various regions of Poland including all voivodships. The data regarding the degree of relatedness among individuals in the sample were not collected. Typings were supported by public funds as a part of the Polish National Program for Transplant Medicine Development. HLA frequency data are available in the Allele Frequencies Net Database.

Keywords: Genetic diversity; HLA alleles; HLA haplotype frequencies; NGS typing; Population genetics.

MeSH terms

Bone Marrow Transplantation
Gene Frequency / genetics*
Genetics, Population*
HLA Antigens / genetics*
Haplotypes
High-Throughput Nucleotide Sequencing
Histocompatibility Testing
Humans
Poland
Registries
Tissue Donors

Substances

HLA Antigens