Inhibiting CtBP2 expression blocks development of esophageal squamous cell carcinoma through decreasing angiogenesis

Yun Jiang; Jianle Chen; Yongfeng Shao

Inhibiting CtBP2 expression blocks development of esophageal squamous cell carcinoma through decreasing angiogenesis

Int J Clin Exp Pathol. 2018 Jun 1;11(6):2990-2999. eCollection 2018.

Authors

Yun Jiang^{1

2}, Jianle Chen³, Yongfeng Shao¹

Affiliations

¹ Department of Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province People's Hospital Nanjing 210029, Jiangsu, People's Republic of China.
² Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University Nantong, Jiangsu, People's Republic of China.
³ Department of Cardiothoracic Surgery, The First People's Hospital of Suqian #120 Suzhi Road, Suqian 223800, People's Republic of China.

PMID: 31938424
PMCID: PMC6958089

Abstract

The aim of this study is to explain the effects and mechanism of CtBP2 in the development of esophageal squamous cell carcinoma. In this study, we first evaluated CtBP2 protein expression of ESCC tumor and adjacent normal tissues by immunohistochemistry (IHC) and Western blot (WB) assay. Meanwhile, the number of vessels of ESCC and adjacent normal tissues were measured by immunofluorescence. In cell experiments, the effects of CtBP2 were evaluated by wound healing assay, flow cytometry detection, and EPC tube formation. The mechanisms of CtBP2 were investigated by immunofluorescence, qRT-PCR, WB, and EdU incorporation assay. In conclusion, CtBP2 inhibits ESCC in vitro and CtBP2 has a key role in the development of ESCC.

Keywords: CtBP2; ESCC; biological activity.