Amperometric Detection of dsDNA using an Acridine-Orange-Modified Glucose Oxidase

Chempluschem. 2017 Nov;82(11):1310. doi: 10.1002/cplu.201700373. Epub 2017 Oct 10.

Abstract

Invited for this month's cover is the group of Prof. Dr. Wolfgang Schuhmann, Dr. Daliborka Jambrec and Dr. Adrian Ruff at Ruhr-Universität in Bochum, Germany. The cover picture shows a novel procedure for the preferential post-hybridization labeling of double-stranded DNA based on the intercalating compound acridine orange, which was covalently bound to glucose oxidase. Labeling with a highly active biocatalyst allows for a simple and sequence-independent amplification of the signal proportional to the amount of hybridized DNA that may be coupled with other amplification strategies. Read the full text of the article at 10.1002/cplu.201700279.