MiR-21 suppresses ox-LDL-induced HUVECs apoptosis by targeting PDCD4

Xiaona Xu; Yan Chen; Zhao Xu; Xue Liang; Xinghua Wang; Yue Zhang; Meng Yuan; Yanping Ni; Hongmei Liu; Guangping Li

MiR-21 suppresses ox-LDL-induced HUVECs apoptosis by targeting PDCD4

Int J Clin Exp Pathol. 2017 Sep 1;10(9):10075-10084. eCollection 2017.

Authors

Xiaona Xu¹, Yan Chen¹, Zhao Xu¹, Xue Liang¹, Xinghua Wang¹, Yue Zhang¹, Meng Yuan¹, Yanping Ni¹, Hongmei Liu¹, Guangping Li¹

Affiliation

¹ Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease, Department of Cardiology, Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University Tianjin, China.

PMID: 31966898
PMCID: PMC6965965

Abstract

This study was to investigate the effects of microRNA-21 (miR-21) on ox-LDL-induced HUVECs apoptosis. MTT assay was performed to evaluate the proliferation of HUVECs. Quantitative RT-PCR was conducted to quantify the expression of miR-21. Western blotting was used to determine protein expression. Annexin V/propidium iodide double staining was adopted to detect cell apoptosis. We found that ox-LD significantly induced HUVECs apoptosis and reduced miR-21 expression. MiR-21 mimic attenuated the apoptosis of HUVECs under ox-LDL treatment compared with the NC groups while miR-21 inhibitors promoted that of HUVECs. MiR-21 directly targeted PDCD4 in HUVECs. Moreover, miR-21 significantly regulated the downstream apoptotic proteins like bax, bad, bcl-2 and caspase3, meanwhile it enhanced the phosphorylation of ERK.

Keywords: HUVECs; PDCD4; apoptosis; microRNA-21; ox-LDL.