Anther Culture in Eggplant (Solanum melongena L.)

Methods Mol Biol. 2020:2122:283-293. doi: 10.1007/978-1-0716-0342-0_20.

Abstract

For a long time, conventional breeding methods have been used to obtain pure, 100% homozygous lines for hybrid seed production in crops of agronomic interest. However, by doubled haploid technology, it is possible to produce 100% homozygous plants derived from precursors of male gametophytes (androgenesis), to accelerate the production of pure lines, which implies important time and cost savings. In this chapter, a protocol for anther culture in eggplant is described, from donor plant growth conditions to regeneration and acclimation of doubled haploid plants, as well as a description of how to analyze ploidy levels of regenerated plants.

Keywords: Androgenesis; Doubled haploid; Embryogenesis; Haploid; Tissue culture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Plant / genetics
  • Haploidy
  • Plant Breeding / methods
  • Plant Development
  • Ploidies
  • Pollen / genetics
  • Pollen / growth & development
  • Solanum melongena / genetics
  • Solanum melongena / growth & development*
  • Tissue Culture Techniques / methods

Substances

  • DNA, Plant