Identification and characterization of a novel variant in C-terminal region of Antithrombin (Ala427Thr) associated with type II AT deficiency leading to polymer formation

J Thromb Thrombolysis. 2020 Oct;50(3):678-685. doi: 10.1007/s11239-020-02048-0.

Abstract

Antithrombin (AT) deficiency is a rare but strong risk factor for the thrombosis development. Mutations in gene encoding AT (SERPINC1) have provided a detailed understanding of AT deficiency and subsequent development of thrombotic complications. In the present study, we describe a case of thrombotic patient with reduced AT activity and normal AT antigen levels. AT deficiency in the patient was explained by the presence of heterozygous mutation g.13397A>G (Ala427Thr) in exon 6 of SERPINC1. Reduced APTT and TT with normal PT were observed. The mutation was found to be absent in healthy controls (n = 62). In vitro purification and characterization of variant AT showed significant decrease in fluorescence emission intensity, decreased bis-ANS fluorescence emission, changes in secondary structure and presence of polymerized AT in patient's plasma as assessed by fluorescence, circular dichroism and transmission electron microscopy respectively. Furthermore, molecular dynamics simulation studies showed altered conformation due to Ala427Thr substitution. Our study shows that genetic screening should be carried out in AT deficient patients in addition to the routinely used functional assays to understand the molecular basis of disease development.

Keywords: Antithrombin; Molecular dynamics simulation; Mutations; Polymerization; Thrombosis.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Antithrombin III / chemistry
  • Antithrombin III / genetics*
  • Blood Coagulation
  • Female
  • Humans
  • Molecular Dynamics Simulation
  • Point Mutation
  • Protein Conformation
  • Thrombophilia / blood
  • Thrombophilia / genetics*

Substances

  • SERPINC1 protein, human
  • Antithrombin III

Supplementary concepts

  • Antithrombin deficiency type 2