Differentiation of Mannheimia haemolytica genotype 1 and 2 strains by visible phenotypic characteristics on solid media

Emily L Wynn; Gennie Schuller; John D Loy; Aspen M Workman; Tara G McDaneld; Michael L Clawson

doi:10.1016/j.mimet.2020.105877

Differentiation of Mannheimia haemolytica genotype 1 and 2 strains by visible phenotypic characteristics on solid media

J Microbiol Methods. 2020 Apr:171:105877. doi: 10.1016/j.mimet.2020.105877. Epub 2020 Feb 20.

Authors

Emily L Wynn¹, Gennie Schuller¹, John D Loy², Aspen M Workman¹, Tara G McDaneld¹, Michael L Clawson³

Affiliations

¹ United States Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, NE, USA.
² University of Nebraska-Lincoln, Institute of Agriculture and Natural Resources, School of Veterinary Medicine and Biomedical Sciences, Lincoln, NE, USA.
³ United States Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, NE, USA. Electronic address: mike.clawson@usda.gov.

PMID: 32088258
DOI: 10.1016/j.mimet.2020.105877

Abstract

Genotype 2 Mannheimia haemolytica associate with the lungs of cattle with bovine respiratory disease more frequently than genotype 1 strains. Different colony colors and morphologies were identified between genotype 1 and 2 solid media cultures. Genotype of strains, and frequency differences between them in mixed cultures are discernable by visual inspection.

Published by Elsevier B.V.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bacterial Typing Techniques / methods*
Cattle
Cattle Diseases / microbiology
Culture Media / pharmacology
Genotype
Mannheimia haemolytica / classification*
Mannheimia haemolytica / genetics*
Mannheimia haemolytica / growth & development
Pasteurellosis, Pneumonic / microbiology*

Substances

Culture Media