CRISPR system: Discovery, development and off-target detection

Shengmiao Chen; Yufeng Yao; Yanchun Zhang; Gaofeng Fan

doi:10.1016/j.cellsig.2020.109577

CRISPR system: Discovery, development and off-target detection

Cell Signal. 2020 Jun:70:109577. doi: 10.1016/j.cellsig.2020.109577. Epub 2020 Feb 22.

Authors

Shengmiao Chen¹, Yufeng Yao¹, Yanchun Zhang¹, Gaofeng Fan²

Affiliations

¹ School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
² School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. Electronic address: fangf@shanghaitech.edu.cn.

PMID: 32097710
DOI: 10.1016/j.cellsig.2020.109577

Abstract

As a revolutionary gene editing tool based on the adaptive immune defense mechanism of bacteria and archaea against exogenous DNA invasion, CRISPR/Cas system shows many remarkable characteristics over ZFNs and TALENs. However, off-target effect remains as one of the major imperfection of CRISPR/Cas system, hindering its further application in translational research. In this review, we highlight major breakthrough cross the development/application of this powerful toolkit, and summarize feasible methods for detecting potential off-target effects during genetic manipulation. We hope this review will assist scientists for accurate genomic editing in their future research.

Keywords: CRISPR; Cas9; Genome editing; Off-target.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Archaea / genetics*
Bacteria / genetics*
CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats*
DNA, Archaeal
DNA, Bacterial
Gene Editing*

Substances

DNA, Archaeal
DNA, Bacterial