Rationale and design of the granulocyte-macrophage colony stimulating factor in peripheral arterial disease (GPAD-3) study

Contemp Clin Trials. 2020 Apr:91:105975. doi: 10.1016/j.cct.2020.105975. Epub 2020 Mar 4.

Abstract

Background: Lower extremity peripheral arterial disease (PAD) is a public health problem and many patients with PAD experience claudication despite adequate medical and/or surgical management. Mobilization of endogenous progenitor cells using Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is a novel therapeutic option that has shown promising results in experimental models and phase I/IIA clinical trials. The GPAD-3 trial will study the effect of two successive administrations of GM-CSF at 3-month interval for improving claudication among patients with lower extremity PAD.

Methods: We plan to recruit 176 patients in this ongoing randomized, double-blind, placebo-controlled Phase IIB trial. After screening for inclusion and exclusion criteria, eligible subjects undergo a 4-week screening phase where they perform subcutaneous placebo injections thrice weekly and walk at least three times a day until they develop claudication. After the screening phase, eligible subjects undergo baseline testing and are randomized 2:1 to receive 500 μg/day of GM-CSF subcutaneously thrice weekly for three weeks or placebo injections. After 3 months, follow-up endpoint testing is performed and subjects in the GM-CSF group receive the second administration of the drug for three weeks while subjects in placebo group receive matching placebo injections. All participants undergo endpoint testing at six-month and nine-month follow-up. The primary endpoint is change in 6-min walk distance between baseline and 6-month follow-up.

Conclusion: GPAD-3 explores a novel approach to address the need for alternative therapies that can alleviate symptoms among patients with lower extremity PAD. If successful, this study will pave the way for a pivotal Phase III trial.

Trial registration: ClinicalTrials.gov NCT03304821.

Keywords: Angiogenesis; Claudication; GM-CSF; Peripheral artery disease.

Publication types

  • Clinical Trial Protocol
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ankle Brachial Index
  • Clinical Trials, Phase II as Topic
  • Diabetes Mellitus / epidemiology
  • Double-Blind Method
  • Exercise Test
  • Female
  • Granulocyte-Macrophage Colony-Stimulating Factor* / administration & dosage
  • Granulocyte-Macrophage Colony-Stimulating Factor* / adverse effects
  • Granulocyte-Macrophage Colony-Stimulating Factor* / therapeutic use
  • Humans
  • Injections, Subcutaneous
  • Lower Extremity*
  • Male
  • Peripheral Arterial Disease* / epidemiology
  • Peripheral Arterial Disease* / therapy
  • Randomized Controlled Trials as Topic
  • Walking / physiology

Substances

  • Granulocyte-Macrophage Colony-Stimulating Factor

Associated data

  • ClinicalTrials.gov/NCT03304821