MicroRNA-590-3p inhibits invasion and metastasis in triple-negative breast cancer by targeting Slug

Meisi Yan; Leiguang Ye; Xinxin Feng; Runze Shi; Zhen Sun; Zhigao Li; Tong Liu

MicroRNA-590-3p inhibits invasion and metastasis in triple-negative breast cancer by targeting Slug

Am J Cancer Res. 2020 Mar 1;10(3):965-974. eCollection 2020.

Authors

Meisi Yan^{1

2}, Leiguang Ye³, Xinxin Feng¹, Runze Shi⁴, Zhen Sun⁵, Zhigao Li⁴, Tong Liu^{4

6}

Affiliations

¹ Department of Pathology, Harbin Medical University Harbin 150081, China.
² Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center TX 77030, United States.
³ Department of Oncology, Harbin Medical University Cancer Hospital Harbin 150000, China.
⁴ Department of Breast Surgery, Harbin Medical University Cancer Hospital Harbin 150000, China.
⁵ Department of Molecular and Cellular Biology, Baylor College of Medical TX 77030, United States.
⁶ Department of Pathology, The University of Texas MD Anderson Cancer Center TX 77030, United States.

PMID: 32266103
PMCID: PMC7136920

Abstract

miR-590-3p acts as a tumor suppressor in glioblastoma multiform, medulloblastoma, hepatocellular carcinoma, and nephroblastoma. Here, we studied the role of miR-590-3p in triple-negative breast cancer (TNBC). The miR-590-3p levels in TNBC specimens were significantly lower than those in non-TNBC specimens. Overexpression of miR-590-3p significantly inhibited migration and invasion of TNBC cells and lung metastasis in vivo. Interestingly, miR-590-3p decreased the Slug mRNA and protein levels in TNBC cells, and luciferase reporter assay showed that miR-590-3p directly targeted 3'-UTR of Slug in TNBC cells. Importantly, overexpression of Slug reversed the inhibitory effect of miR-590-3p on migration and invasion of TNBC cells. Taken together, miR-590-3p inhibits TNBC migration and invasion by directly targeting Slug, suggesting a potential therapeutic effect of miR-590-3p for TNBC.

Keywords: Slug; Triple-negative breast cancer; cancer metastasis; miR-590-3p.