This study presents the first research related to fungal and bacterial growth within electromagnetic fields generated by three-electrode galvanic cells, with PDA growth medium as an electrolyte. We used galvanic microcells constructed with copper, bismuth and zinc metal bars. The configuration of these electrodes was a fundamental agent in the creation of a maximum inhibition zone and in bismuth ion movement. Fungal strains, such as Aspergillus tubingensis and Rhodotorula mucilaginosa, and the bacterium Micrococcus luteus were used as model organisms.