A novel fluorescent probe for detection of Glutathione dynamics during ROS-induced redox imbalance

Tingting Niu; Guoxing Yin; Ting Yu; Yabing Gan; Chenye Zhang; Juanjuan Chen; Wei Wu; Haimin Chen; Haitao Li; Peng Yin

doi:10.1016/j.aca.2020.02.059

A novel fluorescent probe for detection of Glutathione dynamics during ROS-induced redox imbalance

Anal Chim Acta. 2020 Jun 8:1115:52-60. doi: 10.1016/j.aca.2020.02.059. Epub 2020 Feb 28.

Authors

Tingting Niu¹, Guoxing Yin², Ting Yu², Yabing Gan², Chenye Zhang¹, Juanjuan Chen¹, Wei Wu¹, Haimin Chen³, Haitao Li², Peng Yin⁴

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, Zhejiang, 315211, China.
² Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China.
³ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, Zhejiang, 315211, China. Electronic address: chenhaimin@nbu.edu.cn.
⁴ Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China. Electronic address: yinpeng@hunnu.edu.cn.

PMID: 32370869
DOI: 10.1016/j.aca.2020.02.059

Abstract

Monitoring cellular GSH dynamics is of great value to understand its complex biological functions and related diseases. It still remains challenging to understand the mechanism of GSH dynamics in complex intracellular environment. Herein, a novel fluorescent probe featured with chlorinated coumarin-TCF was exploited for sensing of GSH with high selectivity and high sensitivity. Large fluorescence enhancement at 471 nm was observed upon addition of GSH with large emission distance (Δλ_em = 219 nm). This novel probe was successfully applied to monitor endogenous and exogenous GSH dynamics without interference of other thiols via fluorescence imaging. More importantly, using this probe, low dose reactive oxygen species induced GSH increasement through nuclear factor (erythroid-derived 2)-like 2 (Nrf2) signal pathway in BEL-7402 cells was observed. This novel fluorescent probe has the potential for quantitative monitoring of cellular GSH, which will promote further understanding of its physiological and pathological roles in biological systems.

Keywords: Bioimaging; Chlorinated coumarin-TCF; Fluorescent probe; Glutathione dynamics; Redox imbalance.

MeSH terms

Animals
Cells, Cultured
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Glutathione / analysis
Glutathione / metabolism*
Mice
Molecular Structure
Oxidation-Reduction
RAW 264.7 Cells
Reactive Oxygen Species / metabolism*
Zebrafish

Substances

Fluorescent Dyes
Reactive Oxygen Species
Glutathione