Translational control of breast cancer plasticity

Nat Commun. 2020 May 19;11(1):2498. doi: 10.1038/s41467-020-16352-z.

Abstract

Plasticity of neoplasia, whereby cancer cells attain stem-cell-like properties, is required for disease progression and represents a major therapeutic challenge. We report that in breast cancer cells NANOG, SNAIL and NODAL transcripts manifest multiple isoforms characterized by different 5' Untranslated Regions (5'UTRs), whereby translation of a subset of these isoforms is stimulated under hypoxia. The accumulation of the corresponding proteins induces plasticity and "fate-switching" toward stem cell-like phenotypes. Mechanistically, we observe that mTOR inhibitors and chemotherapeutics induce translational activation of a subset of NANOG, SNAIL and NODAL mRNA isoforms akin to hypoxia, engendering stem-cell-like phenotypes. These effects are overcome with drugs that antagonize translational reprogramming caused by eIF2α phosphorylation (e.g. ISRIB), suggesting that the Integrated Stress Response drives breast cancer plasticity. Collectively, our findings reveal a mechanism of induction of plasticity of breast cancer cells and provide a molecular basis for therapeutic strategies aimed at overcoming drug resistance and abrogating metastasis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions / genetics*
  • Antineoplastic Agents / pharmacology
  • Breast Neoplasms / drug therapy
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Cell Hypoxia
  • Cell Line, Tumor
  • Eukaryotic Initiation Factor-2 / genetics
  • Eukaryotic Initiation Factor-2 / metabolism
  • Female
  • Gene Expression Regulation, Neoplastic / drug effects
  • Gene Expression Regulation, Neoplastic / genetics*
  • Humans
  • MCF-7 Cells
  • Nanog Homeobox Protein / genetics
  • Nodal Protein / genetics
  • Phosphorylation / drug effects
  • Protein Biosynthesis / genetics*
  • RNA Isoforms / genetics*
  • Snail Family Transcription Factors / genetics

Substances

  • 5' Untranslated Regions
  • Antineoplastic Agents
  • Eukaryotic Initiation Factor-2
  • Nanog Homeobox Protein
  • Nodal Protein
  • RNA Isoforms
  • SNAI1 protein, human
  • Snail Family Transcription Factors