Establishment of a 7-miRNA-Based Risk Score System for Predicting Prognosis of Pancreatic Cancer

Pancreas. 2020 May/Jun;49(5):655-662. doi: 10.1097/MPA.0000000000001542.

Abstract

Objective: This study developed a prognosis-associated miRNA (PAM)-based risk score system to predict overall survival for pancreatic cancer.

Methods: We screened potential PAMs using bioinformatics technology. A risk score system integrating the PAMs was established, and the predictive value was evaluated. The targets of these PAMs were identified and functional enrichment analysis was performed.

Results: Seven PAMs (hsa-mir-188, hsa-mir-1301, hsa-mir-424, hsa-mir-5010, hsa-mir-584, hsa-mir-5091, and hsa-mir-3613) were identified. We also developed a risk score system, which showed a high Harrell concordance index (C-index, 0.723) for overall survival in the Cancer Genome Atlas data sets. The areas under the curve of the receiver operating characteristic curve at the 1-, 2-, and 3-year survival points were 0.718, 0.832, and 0.903, respectively. In addition, both the C-index and the areas under the curve for recurrence-free survival showed a good outcome, indicating that the system had a satisfactory predictive power. Furthermore, 49 target genes of PAMs were identified. Functional enrichment analysis revealed that these targets may be involved in various biological pathways, including the transforming growth factor β signaling pathway, Notch signaling, and downregulation of SMAD2/3.

Conclusions: The findings of this study suggest that the 7-miRNA-based risk score system is a promising prognostic model for pancreatic cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / genetics*
  • Computational Biology / methods*
  • Gene Expression Profiling / methods
  • Gene Expression Regulation, Neoplastic
  • Gene Regulatory Networks
  • Humans
  • Kaplan-Meier Estimate
  • MicroRNAs / genetics*
  • Pancreatic Neoplasms / diagnosis
  • Pancreatic Neoplasms / genetics*
  • Prognosis
  • ROC Curve
  • Risk Factors

Substances

  • Biomarkers, Tumor
  • MicroRNAs