Cloning, nucleotide sequence and characterization of genes encoding naphthalene dioxygenase of Pseudomonas putida strain NCIB9816

Gene. 1988 Dec 20;73(2):355-62. doi: 10.1016/0378-1119(88)90500-8.

Abstract

We have cloned the naphthalene dioxygenase(ND)-coding genes from Pseudomonas putida strain NCIB9816 based on their ability to convert indole to indigo. The region coding for this enzyme activity was sequenced and three successive open reading frames were found. The corresponding gene products were identified using the T7 polymerase/promoter system. All of them are necessary for the ND activity. A comparison of the ND-coding genes with the ones coding for benzene dioxygenase revealed significant homology which was more pronounced at the nucleotide level than at the amino acid level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular / methods*
  • Dioxygenases
  • Genes*
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Multienzyme Complexes / genetics*
  • Oxygenases / genetics*
  • Plasmids
  • Pseudomonas / enzymology
  • Pseudomonas / genetics*
  • Restriction Mapping

Substances

  • Multienzyme Complexes
  • Oxygenases
  • Dioxygenases
  • naphthalene dioxygenase

Associated data

  • GENBANK/M23914