Abstract
The transcription factor MYB15 plays an important role in grape basal immunity, and its promoter can be used as a potential target in resistance breeding. However, the regulatory mechanisms of cis-elements in its promoter region under a variety of stresses remain unclear. In this study, we identified some putative cis-regulatory elements present upstream of MYB15 in Vitis quinquangularis Shanyang (pVqMYB15_SY) and subsequently characterized the function of these elements using reporter assays. Our results showed that TCA-elements 1 and 2, ABRE, MYC and 3-AF1 binding site 1 are key cis-regulatory elements in pVqMYB15_SY and play important roles in plant bio/abiotic stress resistance.
Keywords:
MYB15 promoter; GUS gene; Grapevine (Vitis quinquangularis); functional analysis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Gene Expression Regulation, Plant / genetics
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Gene Expression Regulation, Plant / physiology
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Plant Proteins / genetics
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Plant Proteins / metabolism*
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Promoter Regions, Genetic / genetics*
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Vitis / genetics*
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Vitis / physiology*
Substances
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Plant Proteins
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Transcription Factors
Grants and funding
This work was supported by the National Natural Science Foundation of China [31970348]; National Natural Science Foundation of China [31600256]; Young Talent fund of University Association for Science and Technology in Shaanxi Province of China [20190207]; Scientific Research Program Funded by Shaanxi Provincial Education Department [18JK0769]; Natural Science Basic Research Plan in Shaanxi Province of China [2017JQ3005]; Northwest University Training Program of Innovation and Entrepreneurship for Undergraduate [2020326]; Young Academic Talent Support Program of Northwest University.