Association of increased diabetes related complication and higher glycemic status in patients with type 2 diabetes mellitus (DM) has been well recognized. This cross sectional study was carried out to assess serum zinc (Zn) level and its relationship to glycemic status in type 2 DM patients and conducted in the Department of Physiology, Dhaka Medical College, Dhaka, Bangladesh from July 2014 to June 2015. Fifty (50) type 2 diabetic subjects with age ranging from 40 to 55 years were study group and fifty healthy subjects matched by ages and BMI were control group. Study population was chosen from Bangladesh Institute of Research for Diabetic Endocrine and Metabolic Disorders (BIRDEM) General Hospital, Dhaka, Bangladesh. Glycated hemoglobin (HbA1c) and fasting serum glucose (FSG) levels were estimated in the laboratory of the Department of Biochemistry, BIRDEM General Hospital, Dhaka, Bangladesh. Serum Zn level was estimated by flame atomic absorption spectrophotometry. For statistical analysis unpaired Student's 't' test was performed. In this study, mean serum zinc level was significantly (p<0.001) lower in patients than that of control group. On correlation analysis, serum Zn level showed significant negative correlation with FSG and HbA1C levels in type 2 diabetic patients. The results conclude that serum Zn level was reduced in DM which was higher with glycemic status of this disease.