Abstract Single cell sequencing technology is different from traditional sequencing method, which is based on population cell average level. It has been widely used in many fields and made great progress in the application of malignant hematological diseases. In this review, the principle, methodology and application of single cell sequencing technology in malignant hematological diseases are summarized briefly, including the study of the pathogenesis in myelodysplastic syndrome, the mechanism of transformation into leukemia, accurate diagnosis and classification, differential diagnosis, evaluation of targeted drug therapeutic efficacy and exploration of biomarkers; specific diagnostic indicators for myeloproliferative diseases, progression of disease monitoring and epidemiological studies; moreover, the pathogenesis and drug resistance of acute myeloid leukemia (AML), which can provide reference for the diagnosis and research of malignant hematological diseases.
题目: 单细胞测序技术在恶性血液病诊疗中的应用.
摘要: 摘要 单细胞测序技术与基于群体细胞平均水平的传统测序方法不同，已经在多个领域得到广泛应用，在恶性血液病中的应用也取得很大进展。本文对单细胞测序技术的原理、方法学以及在多种恶性血液病的应用进行综述，涉及到对骨髓增生异常综合征发病机制、转白机制、精确诊断与分型、鉴别诊断、靶向药疗效评价、生物标志物的探索；以及骨髓增生性疾病的特异性诊断指标的确立、疾病进展监测、流行病学相关研究；还有急性髓系白血病的发病机制以及耐药机制研究，为恶性血液病的诊断和研究提供参考.