Flow cytometric identification of Tfh13 cells in mouse and human

Jennifer S Chen; Jessica D S Grassmann; Uthaman Gowthaman; Sam J Olyha; Tregony Simoneau; M Cecilia Berin; Stephanie C Eisenbarth; Adam Williams

doi:10.1016/j.jaci.2020.04.063

Flow cytometric identification of T_fh13 cells in mouse and human

J Allergy Clin Immunol. 2021 Feb;147(2):470-483. doi: 10.1016/j.jaci.2020.04.063. Epub 2020 Jul 21.

Authors

Jennifer S Chen¹, Jessica D S Grassmann², Uthaman Gowthaman¹, Sam J Olyha¹, Tregony Simoneau³, M Cecilia Berin⁴, Stephanie C Eisenbarth¹, Adam Williams⁵

Affiliations

¹ Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Conn; Department of Immunobiology, Yale University School of Medicine, New Haven, Conn.
² Jackson Laboratory for Genomic Medicine, Farmington, Conn.
³ Department of Pediatrics, Harvard Medical School, Boston, Mass; Division of Respiratory Diseases, Boston Children's Hospital, Boston, Mass.
⁴ Jaffe Food Allergy Institute/Immunology Institute, Icahn School of Medicine at Mount Sinai, New York.
⁵ Jackson Laboratory for Genomic Medicine, Farmington, Conn; Department of Genetics and Genome Sciences, University of Connecticut Health Center, Farmington, Conn. Electronic address: adam.williams@jax.org.

Abstract

Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper T_fh13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying T_fh13 cells in both mice and humans.

Keywords: IL-13; IL-4; IL-5; IgE; T follicular helper cells; T(fh)13 cells; allergy; intracellular cytokine staining.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Cell Separation / methods*
Flow Cytometry / methods*
Humans
Mice
T Follicular Helper Cells*
T-Lymphocyte Subsets*

Abstract

Publication types

MeSH terms

Grants and funding