Response surface methodology based optimization and scale-up production of amylase from a novel bacterial strain, Bacillus aryabhattai KIIT BE-1

Sanjay Kumar Ojha; Puneet Kumar Singh; Snehasish Mishra; Ritesh Pattnaik; Shubha Dixit; Suresh K Verma

doi:10.1016/j.btre.2020.e00506

Response surface methodology based optimization and scale-up production of amylase from a novel bacterial strain, Bacillus aryabhattai KIIT BE-1

Biotechnol Rep (Amst). 2020 Jul 15:27:e00506. doi: 10.1016/j.btre.2020.e00506. eCollection 2020 Sep.

Authors

Sanjay Kumar Ojha^{1

2}, Puneet Kumar Singh¹, Snehasish Mishra¹, Ritesh Pattnaik¹, Shubha Dixit³, Suresh K Verma¹

Affiliations

¹ School of Biotechnology, Kalinga Institute of Industrial Technology (KIIT) Deemed-to-be-University, Bhubaneswar, 751 024, India.
² Pandorum Technologies Pvt. Ltd., Bangalore Bioinnovation Centre, Helix Biotech Park, Electronic City Phase 1, Bengaluru, 560 100, India.
³ School of Pharmacy, Lloyd Institute of Management and Technology, PlotNo.11, Knowledge Park II, Greater Noida, 201310, India.

Abstract

A novel strain KIIT BE-1 isolated from a specialized environment, screened through starch iodine test from a set of eighty-five biodigestate isolates, produced amylase maximally when cultured for 48 h at 37 °C. The molecular and biochemical characterization confirmed it as a strain of Bacillus aryabhattai. It exhibited optimal amylase activity (3.20 U/ml) at 36 h post incubation with a media combination of starch and yeast extract for C-N source respectively. Statistical optimisation by response surface modeling showed R² values of 0.9645 for biomass and 0.9831 for amylase activity, suggesting the significance of the model. The optimised medium (10.25 % starch, 5.0 % peptone, 5.18 % yeast extract, pH 7.3) enhanced the enzyme activity to 4.16 U/ml (1.39-fold) from 3.20 U/ml of un-optimised medium. Further, the biomass yield and the enzymatic activity in optimized medium and process conditions increased by 1.14 and 1.21 folds subjected to a 5 l scaled-up operation in a lab-scale bioreactor.

Keywords: Amylase; Bacillus; Batch fermentation; GenBank; KIIT BE-1; Response surface methodology.