Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi005-A) from a Wilson's disease patient harboring a homozygous Pro992Leu mutation in ATP7B gene

Liting Wei; Jiwei Zhang; Dingbang Chen; Li Feng; Chao Wu; Rui Wang; Xunhua Li

doi:10.1016/j.scr.2020.101909

Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi005-A) from a Wilson's disease patient harboring a homozygous Pro992Leu mutation in ATP7B gene

Stem Cell Res. 2020 Jul 15:47:101909. doi: 10.1016/j.scr.2020.101909. Online ahead of print.

Authors

Liting Wei¹, Jiwei Zhang², Dingbang Chen³, Li Feng³, Chao Wu³, Rui Wang⁴, Xunhua Li⁵

Affiliations

¹ Department of Neurology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 467500, China.
² Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan 450052, China. Electronic address: jiweizhang26@gmail.com.
³ Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
⁴ Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, China.
⁵ Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China. Electronic address: lixunhua26@163.com.

PMID: 32745714
DOI: 10.1016/j.scr.2020.101909

Abstract

Using non-integrative reprogramming method, a human induced pluripotent stem cell (iPSC) line, ZZUNEUi005-A, was generated from a 36-year-old male patient with Wilson's disease, carrying a homozygous Pro992Leu mutation in the ATP7B gene. This cell line shows pluripotency both in vitro and in vivo and has a normal karyotype. Furthermore, we showed that this iPSC line could be differentiated into neural and hepatocyte-like cells.