Investigation of a Sensing Strategy Based on a Nucleophilic Addition Reaction for Quantitative Detection of Bisulfite (HSO3-)

Keith R Everitt; Hannah C Schmitz; Amanda Macke; Jinqing Shan; Eunju Jang; Brandon E Luedtke; Kimberly A Carlson; Haishi Cao

doi:10.1007/s10895-020-02599-8

Investigation of a Sensing Strategy Based on a Nucleophilic Addition Reaction for Quantitative Detection of Bisulfite (HSO₃^-)

J Fluoresc. 2020 Sep;30(5):977-983. doi: 10.1007/s10895-020-02599-8. Epub 2020 Aug 6.

Authors

Keith R Everitt¹, Hannah C Schmitz¹, Amanda Macke², Jinqing Shan¹, Eunju Jang¹, Brandon E Luedtke², Kimberly A Carlson², Haishi Cao³

Affiliations

¹ Department of Chemistry, University of Nebraska, Kearney, NE, 68849, USA.
² Department of Biology, University of Nebraska, Kearney, NE, 68849, USA.
³ Department of Chemistry, University of Nebraska, Kearney, NE, 68849, USA. Caoh1@unk.edu.

Abstract

A reaction-based sensor (NAS-1) showed a high affinity and sensitivity to HSO3- via a nucleophilic addition reaction in the aqueous media, giving dual signals from absorption and emission spectra. NAS-1 was successfully applied in RK13 epithelial cells to detect HSO3- in a cellular environment.

Keywords: 1,8-naphthalimide; Bisulfite; Sensor.

MeSH terms

Animals
Cells, Cultured
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Molecular Structure
Naphthalimides / chemical synthesis
Naphthalimides / chemistry*
Optical Imaging
Rabbits
Spectrometry, Fluorescence
Sulfites / analysis*

Substances

Fluorescent Dyes
Naphthalimides
Sulfites
hydrogen sulfite

Abstract

MeSH terms

Substances

Grants and funding