In-depth virological assessment of kidney transplant recipients with COVID-19

Am J Transplant. 2020 Nov;20(11):3162-3172. doi: 10.1111/ajt.16251. Epub 2020 Sep 12.

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread widely, causing coronavirus disease 2019 (COVID-19) and significant mortality. However, data on viral loads and antibody kinetics in immunocompromised populations are lacking. We aimed to determine nasopharyngeal and plasma viral loads via reverse transcription-polymerase chain reaction and SARS-CoV-2 serology via enzyme-linked immunosorbent assay and study their association with severe forms of COVID-19 and death in kidney transplant recipients. In this study, we examined hospitalized kidney transplant recipients with nonsevere (n = 21) and severe (n = 19) COVID-19. SARS-CoV-2 nasopharyngeal and plasma viral load and serological response were evaluated based on outcomes and disease severity. Ten recipients (25%) displayed persistent viral shedding 30 days after symptom onset. The SARS-CoV-2 viral load of the upper respiratory tract was not associated with severe COVID-19, whereas the plasma viral load was associated with COVID-19 severity (P = .010) and mortality (P = .010). All patients harbored antibodies during the second week after symptom onset that persisted for 2 months. We conclude that plasma viral load is associated with COVID-19 morbidity and mortality, whereas nasopharyngeal viral load is not. SARS-CoV-2 shedding is prolonged in kidney transplant recipients and the humoral response to SARS-CoV-2 does not show significant impairment in this series of transplant recipients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Antibodies, Viral / immunology*
  • COVID-19 / epidemiology
  • COVID-19 / virology*
  • Comorbidity
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • France / epidemiology
  • Humans
  • Kidney Transplantation*
  • Male
  • Middle Aged
  • Nasopharynx / virology
  • Pandemics*
  • SARS-CoV-2 / immunology*
  • Survival Rate / trends
  • Viral Load*

Substances

  • Antibodies, Viral