On-line monitoring of repeated copper pollutions using sediment microbial fuel cell based sensors in the field environment

Abstract

Most microbial fuel cells (MFCs) based sensors rely on exoelectrogenic bacteria to sense contaminants. However, these sensors cannot monitor repeated pollutions unless the exoelectrogenic bacteria are recovered or re-inoculated. To overcome this drawback, a novel sediment microbial fuel cell (SMFC) based sensor was developed for online and in situ monitoring of repeated Cu²⁺ shocks to the overlaying water of paddy soil. The SMFC sensor was operated for a period of eight months in the field environment and a group of CuCl₂ solutions ranging from 12.5 to 400 mg L^-1 Cu²⁺ were repeatedly applied on sunny and rainy days in different seasons. Results show that the SMFC sensor generates one voltage peak in less than 20 s after each Cu²⁺ shock, regardless of the seasons and weather conditions, and the voltage increments from baseline to peak exhibit linear correlation (R² > 0.92) with the logarithm of Cu²⁺ concentrations. Repeated Cu²⁺ pollutions do not decrease the baseline voltage, indicating that the activity of exoelectrogenic bacteria was not significantly inhibited. Soil adsorbed and inactivated approximately 99% of total Cu²⁺. Only 1% of total Cu²⁺ was the toxic exchangeable fraction, of which the concentrations were 0.73, 0.23, and 0.22 mg kg^-1 in the surface (0-3 cm), middle (3-6 cm), and bottom (6-11 cm) layers, respectively. The abundance of 16S rRNA gene transcripts of exoelectrogenic bacteria-associated genera is the lowest in the surface layer (2.86 × 10¹¹ copies g^-1) and the highest in the bottom layer (7.99 × 10¹¹ copies g^-1). Geobacter, Clostridium, Anaeromyxobacter, and Bacillus are the most active exoelectrogenic bacteria-associated genera in the soil. This study suggests that the SMFC sensor could be applied in wetlands to monitor the repeated discharge of Cu²⁺ and other heavy metals.

Keywords: Clostridium; Electrical signal; Exoelectrogenic bacteria; Geobacter; SMFC.