Therapeutic effects of interleukin-37 and induced cardiosphere on treating myocardial ischemia-reperfusion injury

Int Immunopharmacol. 2020 Nov:88:106719. doi: 10.1016/j.intimp.2020.106719. Epub 2020 Sep 8.

Abstract

Backgrounds: Myocardial ischemia-reperfusion injury (MI-RI) has many adverse complications with high mortality rate. In the current study, we investigated the therapeutic advantages of delivering Interleukin-37 (IL-37) by induced cardiospheres (iCS), generated from adult skin fibroblasts via somatic reprogramming, in treating the mice model MI-RI.

Methods: The mouse model of MI-RI was established and the iCS cells with IL-37 overexpression (iCS-IL37) were transplanted into the mice via tail-vein injection. Left ventricular (LV) dimensions and LV pressure-volume measurements were assessed by parasternal long-axis echocardiography and hemodynamic assessment. The infarct size was determined by histology analysis. And the inflammatory responses were analyzed by using enzyme-linked immunosorbent assay (ELISA).

Results: The LV function was significantly improved after the iCS-IL37 transplantation when compared to the vehicle control group and iCS group, including the end-systolic pressure and dP/dtMax. Furthermore, the infarct size was significantly decreased after the iCS-IL37 transplantation. The protein levels of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), were down-regulated by the iCS-IL37 transplantation.

Conclusion: The present study indicated that the iCS with IL-37 overexpression had therapeutic effects on the mice model of MI-RI.

Keywords: IL-37; Induced cardiospheres; MI-RI; Myocardial ischemia-reperfusion injury; iCS.

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Cell- and Tissue-Based Therapy*
  • Disease Models, Animal
  • Fibroblasts / chemistry
  • Fibroblasts / cytology
  • Heart / drug effects
  • Hemodynamics / drug effects
  • Inflammation / metabolism
  • Interleukin-1 / pharmacology*
  • Interleukin-1 / therapeutic use
  • Mice, Inbred C57BL
  • Myocardial Reperfusion Injury / drug therapy*
  • Stem Cell Transplantation*
  • Troponin I / blood
  • Ventricular Function, Left / drug effects

Substances

  • Interleukin-1
  • Troponin I