Identification of differentially expressed long non-coding RNAs and mRNAs in orbital adipose/connective tissue of thyroid-associated ophthalmopathy

Genomics. 2021 Jan;113(1 Pt 2):440-449. doi: 10.1016/j.ygeno.2020.09.001. Epub 2020 Sep 9.

Abstract

Extracellular matrix remodeling and orbital adipose/connective tissue expansion are two key features of thyroid-associated ophthalmopathy (TAO). Recent studies have indicated the critical role of long non-coding RNAs (lncRNAs) in the pathogenesis of ocular disorders. However, little is known about the roles of lncRNAs in orbital adipose/connective tissue of TAO. In this study, the profiles of lncRNAs and mRNAs in the orbital adipose/connective tissue of TAO were identified by RNA sequencing. A total of 809 differential lncRNAs and 607 differential mRNAs were identified, among which 52 genes were found to be significantly related to the extracellular matrix. Co-expression network analysis suggested that lncRNAs might regulate extracellular matrix remodeling in orbital adipose/connective tissue of TAO. Additionally, the target genes of lncRNAs involved in the lipid metabolism and cytokine-cytokine receptor interaction were also identified. These results may provide potential regulatory mechanisms of lncRNAs in the orbital adipose/connective tissue of TAO.

Keywords: Long non-coding RNA; Orbital adipose/connective tissue; RNA sequencing; Thyroid-associated ophthalmopathy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / metabolism
  • Adult
  • Connective Tissue / metabolism
  • Eye / metabolism
  • Female
  • Gene Regulatory Networks
  • Graves Ophthalmopathy / genetics*
  • Graves Ophthalmopathy / metabolism
  • Humans
  • Male
  • Middle Aged
  • RNA, Long Noncoding / genetics*
  • RNA, Long Noncoding / metabolism
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism

Substances

  • RNA, Long Noncoding
  • RNA, Messenger