[Investigation of adenosinetriphosphatase activity of rat liver and thymus cell nuclei]

Biokhimiia. 1978 Nov;43(11):2064-8.
[Article in Russian]

Abstract

The activity of ATPase was studied in highly purified rat liver and thymus cell nuclei, HCO3-, CO3(2-) and SO3(2-) stimulated nuclear ATPase in 1.5--2 times. HSO3- did not affect the enzyme activity, and NO3-, J-, ClO4-,F- and SCN- inhibited it. Bicarbonate increased V and decreased Ka for ATP. SCN- inhibited HCO3--ATPase activity non-competitively with respect to HCO3-. Mg2+-ATPase activity did not depend on pH, and HCO3-component of the activity was decreased under alkaline pH. Mg2+, Mn2+ and Co2+ increased the initial ATPase activity and helped its stimulation with HCO3-. Ba2+, Ni2+ and Zn2+ inhibited the ATPase activity, and Ca2+ did not affect it, Nuclear ATPase is sensitive to 2,4-dinitrophenol and DNAase. It is suggested that cell nuclei have their own H+-ATPase differing for some characteristics from mitochondrial H+-ATPase.

Publication types

  • English Abstract

MeSH terms

  • Adenosine Triphosphatases / metabolism*
  • Animals
  • Bicarbonates / pharmacology
  • Cations, Divalent
  • Cell Nucleus / enzymology*
  • Deoxyribonucleases / metabolism
  • Dinitrophenols / pharmacology
  • Hydrogen-Ion Concentration
  • Liver / enzymology*
  • Magnesium / pharmacology
  • Rats
  • Thiocyanates / pharmacology
  • Thymus Gland / enzymology*

Substances

  • Bicarbonates
  • Cations, Divalent
  • Dinitrophenols
  • Thiocyanates
  • Deoxyribonucleases
  • Adenosine Triphosphatases
  • Magnesium