Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization

Zhi Zou; Diana M Mate; Maximilian Nöth; Felix Jakob; Ulrich Schwaneberg

doi:10.1002/chem.202004039

Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization

Chemistry. 2020 Oct 27;26(60):13537. doi: 10.1002/chem.202004039. Epub 2020 Sep 16.

Authors

Zhi Zou^{1

2}, Diana M Mate^{2

3}, Maximilian Nöth^{1

2}, Felix Jakob^{1

2}, Ulrich Schwaneberg^{1

2}

Affiliations

¹ Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany.
² DWI-Leibniz-Institute for Interactive Materials, Forckenbeckstraβe 50, 52074, Aachen, Germany.
³ Current address: Center of Molecular Biology "Severo Ochoa", Universidad Autónoma de Madrid, Nicolás Cabrera 1, 28049, Madrid, Spain.

PMID: 32935378
DOI: 10.1002/chem.202004039

Abstract

Invited for the cover of this issue is Ulrich Schwaneberg and co-workers at RWTH Aachen University and DWI Leibniz-Institut für Interaktive Materialien. The image depicts a loop engineered, and backbone cyclized Staphylococcus aureus sortase A which shows enhanced robustness in site-specific protein and peptide modifications. Read the full text of the article at 10.1002/chem.202002740.

MeSH terms

Aminoacyltransferases* / chemistry
Aminoacyltransferases* / metabolism
Bacterial Proteins* / chemistry
Bacterial Proteins* / metabolism
Cyclization
Cysteine Endopeptidases* / chemistry
Cysteine Endopeptidases* / metabolism
Staphylococcus aureus / enzymology

Substances

Bacterial Proteins
Aminoacyltransferases
sortase A
Cysteine Endopeptidases