γ-PGA hydrogel loaded with cell-free fat extract promotes the healing of diabetic wounds

J Mater Chem B. 2020 Sep 23;8(36):8395-8404. doi: 10.1039/d0tb01190h.

Abstract

Diabetic wounds are one complication of persistent hyperglycemia and lead to neuropathy and vascular lesions in patients. The promotion of angiogenesis plays an important role in wound healing. Cell-free fat extract (Ceffe) is a cell-free fraction isolated from adipose that is enriched with a range of growth factors, the combination of which can synergistically induce angiogenesis. In this study, we prepared a wound dressing by loading Ceffe with the γ-PGA hydrogel (Ceffe-γ-PGA) to promote the healing of wounds in diabetic mice. The viscosity of Ceffe-γ-PGA was 9.2 pa s, and the water retention rate after 6 hours reached 50%. The slow-release effect of the Ceffe-γ-PGA hydrogel was investigated in vitro in PBS, and the cumulative release rate was 97% after 6 days. Water retention and viscosity analyses revealed that Ceffe-γ-PGA provided a moist environment for the wound surface. The therapeutic effect of the Ceffe-γ-PGA hydrogel on wound healing was studied in vivo in type-II diabetic male db/db mice. After 17 days of wound treatment, the wound area ratio of the Ceffe-γ-PGA group was reduced to 2% of the original, and the capillary density of the Ceffe-γ-PGA group reached 33 mm-2 and was 19 mm-2 higher than that of the untreated group. The cell proliferation rate in the Ceffe-γ-PGA group was 37% higher than that in the untreated group. These results support the use of this system as a promising therapeutic strategy for wound healing in patients with diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / chemistry
  • Animals
  • Diabetes Complications / drug therapy*
  • Diabetes Mellitus, Experimental / complications
  • Drug Carriers / chemistry*
  • Female
  • Humans
  • Hydrogels / chemistry*
  • Male
  • Mice
  • Polyglutamic Acid / analogs & derivatives*
  • Polyglutamic Acid / chemistry
  • Skin / drug effects
  • Skin / pathology
  • Tissue Extracts / therapeutic use*
  • Wound Healing / drug effects*

Substances

  • Drug Carriers
  • Hydrogels
  • Tissue Extracts
  • poly(gamma-glutamic acid)
  • Polyglutamic Acid