Tick-Borne Encephalitis: A Differential Pattern of Intrathecal Humoral Immune Response and Inflammatory Cell Composition Compared with Other Viral CNS Infections

Cells. 2020 Sep 25;9(10):2169. doi: 10.3390/cells9102169.

Abstract

To investigate whether and how cerebrospinal fluid (CSF) findings can contribute to distinguish tick-borne encephalitis (TBE) from herpes simplex virus (HSV) and varicella zoster virus (VZV) induced central nervous system (CNS) infections (HSV-I, VZV-I). Chart review and identification of TBE, HSV- I, and VZV-I was carried out, fulfilling the following criteria: (1) clinical signs of encephalitis and/or meningitis, (2) complete CSF analysis and confirmed viral etiology by either PCR or antibody testing in CSF, (3) hospitalized patients, and (4) available brain magnetic resonance imaging (MRI). Fifty-nine patients with 118 CSF/serum pairs were included. These comprised 21 with TBE (35 CSF/serum pairs), 20 (40 CSF/serum pairs) with HSV-I, and 18 (43 CSF/serum pairs) with VZV-I. In contrast to HSV-I and VZV-I, CSF cell differentiation in TBE showed more often an increased (>20%) proportion of granulocytes (p < 0.01) and a more frequent quantitative intrathecal IgM synthesis (p = 0.001 and p < 0.01, respectively), while the second was even more pronounced when follow-up CSF analyses were included (p < 0.001). CSF findings help to distinguish TBE from other viral infections. In cases with CSF pleocytosis and a positive history for a stay in or near an endemic area, TBE antibodies in CSF and serum should be determined, especially if granulocytes in CSF cell differentiation and/or an intrathecal IgM synthesis is present.

Keywords: HSV; TBE; TBEV; VZV; encephalitis; meningitis; tick-borne encephalitis; viral.

MeSH terms

  • Adult
  • Aged
  • Central Nervous System Infections / blood
  • Central Nervous System Infections / cerebrospinal fluid
  • Central Nervous System Infections / diagnosis*
  • Central Nervous System Infections / virology
  • Diagnosis, Differential*
  • Encephalitis, Tick-Borne / blood
  • Encephalitis, Tick-Borne / cerebrospinal fluid
  • Encephalitis, Tick-Borne / diagnosis*
  • Encephalitis, Tick-Borne / virology
  • Female
  • Herpesvirus 3, Human / pathogenicity
  • Humans
  • Immunity, Humoral / genetics
  • Immunity, Humoral / immunology
  • Immunoglobulin M / blood
  • Leukocytosis / blood
  • Leukocytosis / cerebrospinal fluid
  • Leukocytosis / diagnosis
  • Leukocytosis / virology
  • Magnetic Resonance Imaging
  • Male
  • Meningitis / blood
  • Meningitis / cerebrospinal fluid
  • Meningitis / diagnosis*
  • Meningitis / virology
  • Middle Aged
  • Simplexvirus / immunology
  • Simplexvirus / pathogenicity

Substances

  • Immunoglobulin M