Leginon: New features and applications

Anchi Cheng; Carl Negro; Jessica F Bruhn; William J Rice; Sargis Dallakyan; Edward T Eng; David G Waterman; Clinton S Potter; Bridget Carragher

doi:10.1002/pro.3967

Leginon: New features and applications

Protein Sci. 2021 Jan;30(1):136-150. doi: 10.1002/pro.3967. Epub 2020 Nov 3.

Authors

Anchi Cheng¹, Carl Negro¹, Jessica F Bruhn², William J Rice^{3

4}, Sargis Dallakyan¹, Edward T Eng¹, David G Waterman^{5

6}, Clinton S Potter^{1

7}, Bridget Carragher^{1

7}

Affiliations

¹ The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, New York, USA.
² NanoImaging Services, San Diego, CA, USA.
³ Cryo-Electron Microscopy Core, New York University School of Medicine, New York, New York, USA.
⁴ Department of Cell Biology, New York University School of Medicine, New York, New York, USA.
⁵ STFC, Rutherford Appleton Laboratory, Didcot, UK.
⁶ CCP4, Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, UK.
⁷ Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York, USA.

Abstract

Leginon is a system for automated data acquisition from a transmission electron microscope. Here we provide an updated summary of the overall Leginon architecture and an update of the current state of the package. We also highlight a few recent developments to provide some concrete examples and use cases.

Keywords: automation; cryoEM; microED.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Microscopy, Electron, Transmission*
Software*

Abstract

Publication types

MeSH terms

Grants and funding