Candidatus Frankia nodulisporulans sp. nov., an Alnus glutinosa-infective Frankia species unable to grow in pure culture and able to sporulate in-planta

Aude Herrera-Belaroussi; Philippe Normand; Katharina Pawlowski; Maria P Fernandez; Daniel Wibberg; Jörn Kalinowski; Andreas Brachmann; Fede Berckx; Natuschka Lee; Jochen Blom; Adrien C Pozzi; Pascale Fournier; Lorine Bethencourt; Audrey Dubost; Danis Abrouk; Anita Sellstedt

doi:10.1016/j.syapm.2020.126134

Candidatus Frankia nodulisporulans sp. nov., an Alnus glutinosa-infective Frankia species unable to grow in pure culture and able to sporulate in-planta

Syst Appl Microbiol. 2020 Nov;43(6):126134. doi: 10.1016/j.syapm.2020.126134. Epub 2020 Sep 28.

Authors

Aude Herrera-Belaroussi¹, Philippe Normand², Katharina Pawlowski³, Maria P Fernandez², Daniel Wibberg⁴, Jörn Kalinowski⁴, Andreas Brachmann⁵, Fede Berckx³, Natuschka Lee⁶, Jochen Blom⁷, Adrien C Pozzi², Pascale Fournier², Lorine Bethencourt², Audrey Dubost², Danis Abrouk², Anita Sellstedt⁸

Affiliations

¹ Université de Lyon, Université Lyon 1, CNRS, UMR5557, Ecologie Microbienne, INRA, UMR 1418, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France. Electronic address: aude.herrera-belaroussi@univ-lyon1.fr.
² Université de Lyon, Université Lyon 1, CNRS, UMR5557, Ecologie Microbienne, INRA, UMR 1418, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France.
³ Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, Sweden.
⁴ The Center for Biotechnology CeBiTec, Bielefeld University, Universitätsstraße 25, 33615 Bielefeld, Germany.
⁵ Biocenter of the LMU Munich, Genetics Section, Grosshaderner Str. 2-4, D-82152 Planegg-Martinsried, Germany.
⁶ Department of Ecology and Environmental Science, Umeå University, 90187 Umeå, Sweden.
⁷ Bioinformatics & Systems Biology, Justus-Liebig-University, 35392 Giessen, Hesse, Germany.
⁸ Department of Plant Physiology, UPSC, Umeå University, 90187 Umeå, Sweden. Electronic address: anita.sellstedt@umu.se.

PMID: 33059155
DOI: 10.1016/j.syapm.2020.126134

Abstract

We describe a new Frankia species, for three non-isolated strains obtained from Alnus glutinosa in France and Sweden, respectively. These strains can nodulate several Alnus species (A. glutinosa, A. incana, A. alnobetula), they form hyphae, vesicles and sporangia in the root nodule cortex but have resisted all attempts at isolation in pure culture. Their genomes have been sequenced, they are significantly smaller than those of other Alnus-infective species (5Mb instead of 7.5Mb) and are very closely related to one another (ANI of 100%). The name Candidatus Frankia nodulisporulans is proposed. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and draft genome sequences reported in this study for AgTrS, AgUmASt1 and AgUmASH1 are MT023539/LR778176/LR778180 and NZ_CADCWS000000000.1/CADDZU010000001/CADDZW010000001, respectively.

Keywords: Comparative genomics; Frankia-Alnus symbiosis; Hydrogenasee; In-planta sporulation; Sp+ Frankia strains.

MeSH terms

Alnus / microbiology*
Bacterial Typing Techniques
DNA, Bacterial / genetics
France
Frankia / classification*
Frankia / isolation & purification
Phylogeny*
RNA, Ribosomal, 16S / genetics
Root Nodules, Plant / microbiology*
Sequence Analysis, DNA
Sweden

Substances

DNA, Bacterial
RNA, Ribosomal, 16S