Characteristics of internalization of NMDA-type GluRs with antibodies to GluN1 and GluN2B

Yukitoshi Takahashi; Shigeko Nishimura; Emiko Takao; Risa Kasai; Kaoru Enokida; Kuniko Ida; Masataka Fukuoka; Takayoshi Koike; Hiroo Omatsu; Tokito Yamaguchi; Shiho Takano; Tomoyuki Yoshida; Hisashi Mori

doi:10.1016/j.jneuroim.2020.577427

Characteristics of internalization of NMDA-type GluRs with antibodies to GluN1 and GluN2B

J Neuroimmunol. 2020 Dec 15:349:577427. doi: 10.1016/j.jneuroim.2020.577427. Epub 2020 Oct 15.

Authors

Affiliations

¹ National Epilepsy Center, NHO, Shizuoka Institute of Epilepsy and Neurological Disorders, 886 Urushiyama, Aoi-ward, Shizuoka, Japan; Department of Pediatrics, Gifu University School of Medicine, Yanagido, Gifu, Japan; School of Pharmaceutical Sciences, University of Shizuoka, Japan. Electronic address: takahashi-ped@umin.ac.jp.
² National Epilepsy Center, NHO, Shizuoka Institute of Epilepsy and Neurological Disorders, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: 307-seika@mail.hosp.go.jp.
³ National Epilepsy Center, NHO, Shizuoka Institute of Epilepsy and Neurological Disorders, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: takao.emiko.ev@mail.hosp.go.jp.
⁴ National Epilepsy Center, NHO, Shizuoka Institute of Epilepsy and Neurological Disorders, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: 307-yakuri@mail.hosp.go.jp.
⁵ National Epilepsy Center, NHO, Shizuoka Institute of Epilepsy and Neurological Disorders, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: 307-seiri@mail.hosp.go.jp.
⁶ National Epilepsy Center, NHO, Shizuoka Institute of Epilepsy and Neurological Disorders, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: ida.kuniko.qb@mail.hosp.go.jp.
⁷ National Epilepsy Center, NHO, Shizuoka Institute of Epilepsy and Neurological Disorders, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: taka0730@shizuokamind.org.
⁸ National Epilepsy Center, NHO, Shizuoka Institute of Epilepsy and Neurological Disorders, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: koike.takayoshi.bk@mail.hosp.go.jp.
⁹ National Epilepsy Center, NHO, Shizuoka Institute of Epilepsy and Neurological Disorders, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: hrw-ohmatsu@umin.ac.jp.
¹⁰ National Epilepsy Center, NHO, Shizuoka Institute of Epilepsy and Neurological Disorders, 886 Urushiyama, Aoi-ward, Shizuoka, Japan. Electronic address: tokito@shizuokamind.org.
¹¹ Department of Molecular Neuroscience, Graduate Schools of Innovative Life Science and Medicine, University of Toyama, Toyama, Japan.
¹² Department of Molecular Neuroscience, Graduate Schools of Innovative Life Science and Medicine, University of Toyama, Toyama, Japan. Electronic address: toyoshid@med.u-toyama.ac.jp.
¹³ Department of Molecular Neuroscience, Graduate Schools of Innovative Life Science and Medicine, University of Toyama, Toyama, Japan. Electronic address: hmori@med.u-toyama.ac.jp.

PMID: 33091764
DOI: 10.1016/j.jneuroim.2020.577427

Abstract

To characterize internalization of NMDA-type glutamate receptors (GluRs) by antibodies to NMDA-type GluRs, we produced rabbit antibodies to N-terminals of human GluN1 and GluN2B, and examined internalization of NMDA-type GluRs in HEK293T cells using confocal microscopy. Internalization of NMDA-type GluRs occurred from at least 10 min after incubation with antibodies to GluN1 and or GluN2B and was temperature-dependent. These findings confirm that antibodies to N-terminals of GluN1 and GluN2B present in the cerebrospinal fluid of patients with NMDAR encephalitis can mediate prompt internalization of NMDA-type GluR complexes.

Keywords: Antibodies; GluN1; GluN2B; Internalization; NMDA-type GluR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Autoantibodies / genetics
Autoantibodies / metabolism*
HEK293 Cells
Humans
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism*
Rabbits
Receptors, Glutamate / genetics
Receptors, Glutamate / metabolism
Receptors, N-Methyl-D-Aspartate / genetics
Receptors, N-Methyl-D-Aspartate / metabolism*

Substances

Autoantibodies
GRIN1 protein, human
NR2B NMDA receptor
Nerve Tissue Proteins
Receptors, Glutamate
Receptors, N-Methyl-D-Aspartate