PCSK9 loss of function is protective against extra-coronary atherosclerotic cardiovascular disease in a large multi-ethnic cohort

PLoS One. 2020 Nov 9;15(11):e0239752. doi: 10.1371/journal.pone.0239752. eCollection 2020.

Abstract

Background: Therapeutic inhibition of PCSK9 protects against coronary artery disease (CAD) and ischemic stroke (IS). The impact on other diseases remains less well characterized.

Methods: We created a genetic risk score (GRS) for PCSK9 using four single nucleotide polymorphisms (SNPs) at or near the PCSK9 locus known to impact lower LDL-Cholesterol (LDL-C): rs11583680, rs11591147, rs2479409, and rs11206510. We then used our GRS to calculate weighted odds ratios reflecting the impact of a genetically determined 10 mg/dL decrease in LDL-C on several pre-specified phenotypes including CAD, IS, peripheral artery disease (PAD), abdominal aortic aneurysm (AAA), type 2 diabetes, dementia, chronic obstructive pulmonary disease, and cancer. Finally, we used our weighted GRS to perform a phenome-wide association study.

Results: Genetic and electronic health record data that passed quality control was available in 312,097 individuals, (227,490 White participants, 58,907 Black participants, and 25,700 Hispanic participants). PCSK9 mediated reduction in LDL-C was associated with a reduced risk of CAD and AAA in trans-ethnic meta-analysis (CAD OR 0.83 [95% CI 0.80-0.87], p = 6.0 x 10-21; AAA OR 0.76 [95% CI 0.68-0.86], p = 2.9 x 10-06). Significant protective effects were noted for PAD in White individuals (OR 0.83 [95% CI 0.71-0.97], p = 2.3 x 10-04) but not in other genetic ancestries. Genetically reduced PCSK9 function associated with a reduced risk of dementia in trans-ethnic meta-analysis (OR 0.86 [95% CI 0.78-0.93], p = 5.0 x 10-04).

Conclusions: Genetically reduced PCSK9 function results in a reduction in risk of several important extra-coronary atherosclerotic phenotypes in addition to known effects on CAD and IS, including PAD and AAA. We also highlight a novel reduction in risk of dementia, supporting a well-recognized vascular component to cognitive impairment and an opportunity for therapeutic repositioning.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cholesterol, LDL / blood
  • Cognitive Dysfunction / genetics
  • Coronary Artery Disease / genetics*
  • Coronary Artery Disease / prevention & control*
  • Diabetes Mellitus, Type 2 / genetics
  • Female
  • Genetic Predisposition to Disease / genetics
  • Genome-Wide Association Study
  • Humans
  • Ischemic Stroke / genetics*
  • Ischemic Stroke / prevention & control*
  • Loss of Function Mutation / genetics
  • Male
  • Polymorphism, Single Nucleotide / genetics
  • Proprotein Convertase 9 / genetics*
  • Risk

Substances

  • Cholesterol, LDL
  • PCSK9 protein, human
  • Proprotein Convertase 9