Novel insights into biodegradation, interaction, internalization and impacts of high-aspect-ratio TiO2 nanomaterials: A systematic in vivo study using Drosophila melanogaster

Abstract

The elongated nature of the high-aspect-ratio nanomaterials (NMs) can help us to obtain valuable information on its biodegradation, physical interaction with target-cells, and internalization. Three different length nano-titanium have been studied using Drosophila, TEM, and different biological markers. Nano-titanium, regardless of its shape, was eroded and degraded just entering the gut lumen of the larvae. Results showed that the distinguished shape of nanowires helps to understand the interactions of NMs with the intestinal barrier. The peritrophic membrane, as the first defense line of the intestinal barrier, succeeded in the reservation of NMs, though the perpendicular particles of nanowires stabbing it, making pores, and permitting their translocation into intestinal cells. On the other side, the exposure to TiO₂NPs did not decrease egg-to-adult viability, but all its different shapes, especially nanowires, mediated a wide molecular response including changes of expression in genes involved in stress, antioxidant, repair, and physical interaction responses. All these changes concerning their ability to elevate ROS levels ultimately led to potential genotoxicity. So, the high aspect ratio NMs are efficient in understanding the outstanding issues of NMs exposure, but at the same time could induce genotoxic impact rather than the low aspect ones.

Keywords: Biodegradation; Drosophila melanogaster; Genotoxicity; High aspect ratio; Internalization; Length; Nanomaterials; Nanowires; Oxidative stress; Titanium oxide.