Measuring the Effects of Circadian Rhythm-Related Manipulations on Depression-Like Behavior in Rodents: Forced Swim and Tail Suspension Tests

Chelsea A Vadnie; Lauren M DePoy; Colleen A McClung

doi:10.1007/978-1-0716-0381-9_5

Measuring the Effects of Circadian Rhythm-Related Manipulations on Depression-Like Behavior in Rodents: Forced Swim and Tail Suspension Tests

Methods Mol Biol. 2021:2130:69-78. doi: 10.1007/978-1-0716-0381-9_5.

Authors

Chelsea A Vadnie¹, Lauren M DePoy¹, Colleen A McClung²

Affiliations

¹ Department of Psychiatry, Translational Neuroscience Program, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
² Psychiatry and Clinical and Translational Science, Translational Neuroscience Program, Center for Neuroscience University of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. mcclungca@upmc.edu.

Abstract

The forced swim and tail suspension tests are commonly used to determine the effects of circadian-related pharmacological, genetic, and environmental manipulations on depression-like behavior in rodents. Both tests involve scoring immobility of rodents in an inescapable condition. Here we describe how to set up and carry out these tests.

Keywords: Antidepressant; Depression; Forced swim test; Immobility; Mice; Rats; Tail suspension test.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Circadian Rhythm*
Depression / etiology
Depression / physiopathology*
Hindlimb Suspension / methods*
Hindlimb Suspension / standards
Mice
Physical Conditioning, Animal / methods*
Physical Conditioning, Animal / standards
Swimming

Abstract

Publication types

MeSH terms

Grants and funding