A new hand-held holder optimizes the parameters of the laser-induced choroidal neovascularization model in mice

Bai Xue; Shanshan Zhang; Li Gan; Weifeng Lu; Jie Li

doi:10.1016/j.exer.2020.108392

A new hand-held holder optimizes the parameters of the laser-induced choroidal neovascularization model in mice

Exp Eye Res. 2021 Feb:203:108392. doi: 10.1016/j.exer.2020.108392. Epub 2020 Dec 15.

Authors

Bai Xue¹, Shanshan Zhang¹, Li Gan¹, Weifeng Lu², Jie Li³

Affiliations

¹ Institute of Laboratory Medicine, Sichuan Provincial Key Laboratory for Human, Disease Gene Study, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610054, China.
² Anesthesia Operation Center, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China. Electronic address: 785252430@qq.com.
³ Department of Ophthalmology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China. Electronic address: doctorjacklee@163.com.

PMID: 33338490
DOI: 10.1016/j.exer.2020.108392

Abstract

Background: The laser-induced choroidal neovascularization (CNV) mouse model, as the most classic animal model of age-related macular degeneration (AMD), has been widely used. We designed a hand-held mouse holder to optimize mouse fixation in the laser-induced CNV modelling process, which was inconvenient until now. This study aimed to evaluate the effectiveness of our in-house hand-held mouse holder design in the laser-induced CNV mouse modelling process.

Methods: Six ophthalmic residents were invited to perform laser-induced CNV mouse modelling by hand or using the holder. We compared the learning time of residents and their physical and mental fatigue with the two methods. In addition, we compared the parameters of CNV modelling with two methods by a skilled operator, including the time of photocoagulation, induction rate and uniformity of CNV lesions.

Results: In the learning phase, the average learning time to master the modelling method was significantly shortened by utilizing the holder. The fatigue in the operation process was quantified to a level from 0 to 4, and the physical fatigue by using holder (0.8 ± 0.3) was lower than by hand (2.6 ± 0.4), and the mental fatigue was relieved from 2.3 ± 0.5 to 0.4 ± 0.3. On the other hand, the skilled operator can significantly shorten the time of laser photocoagulation from 146.7 ± 36.0 s to 63.6 + 5.7 s and improve the success rate of modelling from 50.0% ± 8.3%-87.5% ± 6.7% by using a holder compared to hand. In addition, the standard error of the mean (SEM) of the distance between the CNV lesion and the optic nerve (ON) and the distance between each lesion was reduced.

Conclusion: This hand-held mouse holder could optimize the setting and conditions of laser-induced CNV mouse modelling by improving the learning curve, reducing fatigue, shortening the time for photocoagulation, improving the success rate and consistency of laser-induced lesions.

Keywords: Age-related macular degeneration; Fixing tool; Laser-induced choroidal neovascularization; Mouse model.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Angiogenesis Inhibitors / therapeutic use
Animals
Bevacizumab / therapeutic use
Choroidal Neovascularization / diagnosis
Choroidal Neovascularization / drug therapy
Choroidal Neovascularization / etiology*
Disease Models, Animal*
Equipment Design*
Fluorescein Angiography
Humans
Laser Coagulation / adverse effects*
Learning Curve
Male
Mice
Mice, Inbred C57BL
Restraint, Physical / instrumentation*
Restraint, Physical / veterinary
Slit Lamp Microscopy
Vascular Endothelial Growth Factor A / antagonists & inhibitors

Substances

Angiogenesis Inhibitors
Vascular Endothelial Growth Factor A
vascular endothelial growth factor A, mouse
Bevacizumab