Micropatterning of Cells on Gold Surfaces for Biophysical Applications

STAR Protoc. 2020 Sep 17;1(3):100106. doi: 10.1016/j.xpro.2020.100106. eCollection 2020 Dec 18.

Abstract

We developed a reproducible micropatterning method to manipulate and normalize cell shape and cell-cell separation on gold. We used methoxy polyethylene glycol thiol (PEG-SH) to create a self-assembled monolayer that can be oxidized at desired shapes through a photomask with deep UV light. The oxidized PEG can be coated with extracellular matrix proteins and seeded with cells adopting the pre-defined shape. The developed and analyzed surfaces can be used in a wide range of biophysical applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Separation / methods*
  • Cell Shape / physiology
  • Gold / chemistry
  • Polyethylene Glycols / chemistry
  • Sulfhydryl Compounds / chemistry
  • Surface Properties
  • Ultraviolet Rays

Substances

  • Sulfhydryl Compounds
  • Polyethylene Glycols
  • Gold
  • monomethoxypolyethylene glycol