Modifications to the classical rat aortic ring model to allow vascular degeneration studies

Guillaume Blot; Thérèse-Marie Sartoris; Florian Sennlaub; Xavier Guillonneau

doi:10.1016/j.xpro.2020.100281

Modifications to the classical rat aortic ring model to allow vascular degeneration studies

STAR Protoc. 2021 Jan 21;2(1):100281. doi: 10.1016/j.xpro.2020.100281. eCollection 2021 Mar 19.

Authors

Guillaume Blot¹, Thérèse-Marie Sartoris¹, Florian Sennlaub¹, Xavier Guillonneau¹

Affiliation

¹ Institut de la Vision, Sorbonne Université, INSERM, CNRS, Paris, France.

Abstract

The classical aortic ring model is well suited for deciphering pro-angiogenic processes. Here, we propose simple modifications of the standard protocol to study various anti-angiogenic processes from growth arrest to capillary degeneration. Aortic rings are cultured under basal conditions for 6 days to allow physiological vessel sprouting and then split into treatment groups to follow capillary growth or degeneration for an additional 2 days.

Keywords: Cell biology; Cell culture; Model organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aorta / metabolism*
Capillaries / metabolism*
Neovascularization, Physiologic*
Organ Culture Techniques
Rats
Rats, Sprague-Dawley