Restriction Enzymes

Cold Spring Harb Protoc. 2021 Apr 1;2021(4). doi: 10.1101/pdb.top101360.

Abstract

Restriction enzymes provided the foundation on which molecular cloning was built, and they remain as essential tools in current recombinant DNA technology. The three classes of restriction enzymes and their features are introduced here.

MeSH terms

  • Animals
  • Bacteria / enzymology
  • Bacteria / genetics
  • Bacteria / metabolism
  • Binding Sites / genetics
  • Cloning, Molecular / methods*
  • DNA / genetics*
  • DNA / metabolism
  • DNA Restriction Enzymes / classification
  • DNA Restriction Enzymes / metabolism*
  • DNA, Recombinant / genetics*
  • Deoxyribonucleases, Type I Site-Specific / metabolism
  • Deoxyribonucleases, Type II Site-Specific / metabolism
  • Deoxyribonucleases, Type III Site-Specific / metabolism
  • Genetic Vectors / genetics*
  • Humans
  • Plasmids / genetics*
  • Protein Binding
  • Substrate Specificity

Substances

  • DNA, Recombinant
  • DNA
  • DNA Restriction Enzymes
  • Deoxyribonucleases, Type I Site-Specific
  • Deoxyribonucleases, Type II Site-Specific
  • Deoxyribonucleases, Type III Site-Specific