cDNA cloning and sequencing of a new gene intensely expressed in early differentiation stages of embryonal carcinoma cells and in mid-gestation period of mouse embryogenesis

Biochem Biophys Res Commun. 1988 Mar 30;151(3):1312-8. doi: 10.1016/s0006-291x(88)80505-9.

Abstract

By the differential hybridization technique, we isolated a cDNA clone, MK1, whose RNA level increased in early stages of retinoic acid-induced differentiation of embryonal carcinoma cells. The amount of MK1 RNA progressively decreased in the later stages of the differentiation. In mouse embryos, MK1 RNA was abundant in mid-gestation stages (Day 8 to Day 11) and decreased thereafter. The corresponding RNA was 1.0 killobase in size. From the nucleotide sequence, MK1 gene was predicted to code a polypeptide of molecular weight 9,971, which was rich in basic amino acids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Differentiation
  • Cloning, Molecular*
  • DNA / analysis*
  • Embryonic and Fetal Development*
  • Gene Expression Regulation*
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Teratoma / genetics*
  • Teratoma / pathology

Substances

  • DNA

Associated data

  • GENBANK/M19662