Real-Time Confocal Imaging for Hidradenitis Suppurativa: Description of Morphological Aspects and Focus on the Role of Follicular Ostia

Dermatology. 2021;237(5):705-711. doi: 10.1159/000513918. Epub 2021 Feb 15.

Abstract

Background: Hidradenitis suppurativa (HS) is a chronic, recurrent, inflammatory skin disease involving the follicular unit. Growing evidence suggests a crucial role of hyperkeratosis, infundibular plugging and perifolliculitis in its pathogenesis.

Objectives: To characterize the microscopic morphology of open comedos using in vivo reflectance confocal microscopy (RCM) and define alterations occurring in HS normal-appearing perilesional skin compared to healthy skin of a control group, discussing therefore microscopic differences.

Materials and methods: Twenty patients (11 males, 9 females; aged 19-51 years) affected by HS were recruited. RCM was performed on open comedos of axillary/inguinal regions and on normal-appearing skin areas distant at least 1.5 cm from HS lesions. Ten healthy individuals (6 males, 4 females) were included as control group.

Results: RCM aspects of 78 open comedos were explored, observing circular/ovoidal structures disclosing a hyperrefractive amorphous material (72/78, 92.3%) within the infundibular cavity surrounded with a bright (51/78, 65.4%) and regular (52/78, 66.2%) border. Follicular ostia of HS perilesional skin (n = 541) compared to follicular ostia of a control group (n = 321) were characterized by a larger infundibular diameter (183.4 vs. 85.8 µm) and diverged in terms of material collected inside infundibula (44.5 vs. 21%) and keratinization of the border (47.6 vs. 25.5%).

Conclusion: In vivo characterization of open comedos and the recognition of microscopic subclinical alterations of HS normal-appearing skin, compared to healthy skin, could add further insights into the definition of biological events leading to HS manifestations, including the dysregulated process of keratinization.

Keywords: Comedos; Digital imaging; Follicular units; Hidradenitis suppurativa; Reflectance confocal microscopy.

MeSH terms

  • Adult
  • Case-Control Studies
  • Female
  • Hair Follicle / diagnostic imaging*
  • Hair Follicle / pathology*
  • Hidradenitis Suppurativa / diagnostic imaging*
  • Hidradenitis Suppurativa / etiology
  • Hidradenitis Suppurativa / pathology*
  • Humans
  • Male
  • Microscopy, Confocal
  • Middle Aged
  • Young Adult