Optimization of Analytical Procedure for In-hospital Rapid Quantification of Serum Level of Favipiravir in the Pharmacological Treatment of COVID-19

Anal Sci. 2021 Sep 10;37(9):1301-1304. doi: 10.2116/analsci.21N004. Epub 2021 Feb 19.

Abstract

An in-hospital rapid method for quantifying the serum level of favipiravir (FPV) in the pharmacological treatment of COVID-19 was developed by an appropriate combination of a solid-phase extraction treatment and a reversed-phase HPLC/UV detection system. The quantification method was well-validated and applied to measuring the serum FPV level in a clinical practice at a general hospital that accepts COVID-19 patients. Furthermore, an analysis of data from our preliminary interaction analysis revealed, for the first time, that FPV selectively forms complexes with ferric (Fe3+) and cupric (Cu2+) ions.

Keywords: COVID-19; HPLC; In-hospital quantification; favipiravir; ferric ion; serum level; solid-phase extraction.

MeSH terms

  • Amides* / blood
  • Amides* / chemistry
  • Antiviral Agents* / blood
  • Antiviral Agents* / therapeutic use
  • COVID-19 / blood
  • COVID-19 Drug Treatment*
  • Chromatography, High Pressure Liquid / methods
  • Humans
  • Phosphoramides / blood
  • Pyrazines* / blood
  • Pyrazines* / therapeutic use
  • SARS-CoV-2 / isolation & purification
  • Solid Phase Extraction / methods

Substances

  • favipiravir
  • Amides
  • Pyrazines
  • Antiviral Agents
  • Phosphoramides