Differential expression of miR-130a-3p modulate ovarian epithelial carcinoma (OEC) cell development and could be a biomarker for OEC

J Reprod Immunol. 2021 Jun:145:103310. doi: 10.1016/j.jri.2021.103310. Epub 2021 Mar 10.

Abstract

A lot of research investigations, conducted in the recent years, establishes that microRNAs (miRNAs) have an important role in keeping both growth and metastasis of Ovarian Epithelial Carcinoma (OEC) under control. However, the clinical and functional role of miR-130a-3p in OEC is yet to be explored. Through quantitative reaction i.e., qRT-PCR, the expression of miR-130a-3p was assessed in tissues and cell lines of OEC patients. This analysis determined the relationship between the expression of miR-130a-3p and its clinicopathology with the overall survival rate of OEC patients. The author made use of cell counting analysis (CCK8) and in vitro flow cytometry to understand the functional and biological impact of miR-130a-3p expression. In comparison with neighboring normal tissues, the expression of miR-130a-3p was found to be lower in 7 OEC samples. Few reasons are cited for this scenario i.e., low expression of miR-130a-3p, such as low overall OEC patient survival rate, incidence of FIGO and metastasis of lymph nodes. miR-130a-3p has been found as an independent candidate for predicting the prognosis of OEC patients, as per Multivariate Cox research. When miR-130a-3p is over-expressed, as per the enhanced mechanism, it prevents both cell proliferation and cell cycle production in OEC. The current study findings emphasize that miR-130a-3p can be leveraged as a biomarker of prognosis and possibly as a target in the treatment of OEC.

Keywords: Apoptosis; Biomarkers; Cancer immunology; Gynecology; Metastasis; Ovarian epithelial carcinoma (OEC); miR-130a-3p.

Publication types

  • Observational Study
  • Research Support, Non-U.S. Gov't
  • Retracted Publication

MeSH terms

  • Adult
  • Biomarkers, Tumor / analysis
  • Biomarkers, Tumor / metabolism*
  • Carcinoma, Ovarian Epithelial / diagnosis
  • Carcinoma, Ovarian Epithelial / genetics*
  • Carcinoma, Ovarian Epithelial / mortality
  • Carcinoma, Ovarian Epithelial / surgery
  • Cell Cycle / genetics
  • Cell Line, Tumor
  • Cell Proliferation / genetics
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • MicroRNAs / analysis
  • MicroRNAs / metabolism*
  • Middle Aged
  • Ovarian Neoplasms / diagnosis
  • Ovarian Neoplasms / genetics*
  • Ovarian Neoplasms / mortality
  • Ovarian Neoplasms / surgery
  • Ovariectomy
  • Ovary / pathology
  • Ovary / surgery
  • Prognosis
  • Survival Rate

Substances

  • Biomarkers, Tumor
  • MIRN130 microRNA, human
  • MicroRNAs