Molecular-scale axial localization by repetitive optical selective exposure

Lusheng Gu; Yuanyuan Li; Shuwen Zhang; Maoge Zhou; Yanhong Xue; Weixing Li; Tao Xu; Wei Ji

doi:10.1038/s41592-021-01099-2

Molecular-scale axial localization by repetitive optical selective exposure

Nat Methods. 2021 Apr;18(4):369-373. doi: 10.1038/s41592-021-01099-2. Epub 2021 Apr 1.

Authors

Lusheng Gu^#^{1

2

3

4

5}, Yuanyuan Li^#^{1

2

5}, Shuwen Zhang^#^{1

2

4

6}, Maoge Zhou^{1

2

4}, Yanhong Xue^{1

2

4}, Weixing Li^{1

2

3

5}, Tao Xu^{7

8

9

10

11}, Wei Ji^{12

13

14

15

16}

Affiliations

¹ National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
² Physical Science Laboratory, Huairou National Comprehensive Science Center, Beijing, China.
³ Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, Guangdong, China.
⁴ College of Life Science, University of Chinese Academy of Sciences, Beijing, China.
⁵ Core Facility for Protein Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
⁶ School of Future Technology, University of Chinese Academy of Sciences, Beijing, China.
⁷ National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China. xutao@ibp.ac.cn.
⁸ Physical Science Laboratory, Huairou National Comprehensive Science Center, Beijing, China. xutao@ibp.ac.cn.
⁹ Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, Guangdong, China. xutao@ibp.ac.cn.
¹⁰ College of Life Science, University of Chinese Academy of Sciences, Beijing, China. xutao@ibp.ac.cn.
¹¹ School of Future Technology, University of Chinese Academy of Sciences, Beijing, China. xutao@ibp.ac.cn.
¹² National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China. jiwei@ibp.ac.cn.
¹³ Physical Science Laboratory, Huairou National Comprehensive Science Center, Beijing, China. jiwei@ibp.ac.cn.
¹⁴ Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, Guangdong, China. jiwei@ibp.ac.cn.
¹⁵ College of Life Science, University of Chinese Academy of Sciences, Beijing, China. jiwei@ibp.ac.cn.
¹⁶ Core Facility for Protein Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China. jiwei@ibp.ac.cn.

^# Contributed equally.

PMID: 33795876
DOI: 10.1038/s41592-021-01099-2

Abstract

We introduce an axial localization with repetitive optical selective exposure (ROSE-Z) method for super-resolution imaging. By using an asymmetric optical scheme to generate interference fringes, a <2 nm axial localization precision was achieved with only ~3,000 photons, which is an approximately sixfold improvement compared to previous astigmatism methods. Nanoscale three-dimensional and two-color imaging was demonstrated, illustrating how this method achieves superior performance and facilitates the investigation of cellular nanostructures.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Imaging, Three-Dimensional / methods*
Microscopy / methods*
Photons