A static magnetic field changes the expression profile of the transforming growth factor β family genes in human cells that have been treated with fluoride ions

Abstract

One of the molecular pathways that can be modified in cells that are under the influence of fluoride exposure is the transforming growth factor β (TGFβ) signaling pathway. It has also been shown that the effect of static magnetic field on the cellular processes is linked to the activation of many important signal cascades. Therefore, the aim of this study was to evaluate whether the SMF changes the expression profile of TGFβ family genes in NaF-treated human cells. The expression of the genes linked with TGFβ were analyzed using the oligonucleotide microarrays technique and the expression of the TGFβ isoforms was determined using the RT-qPCR and ELISA techniques. Our research showed that SMF modified the activity of the TGFβ-related genes and that their levels are altered by fluoride. This offers hope for planning future therapeutic strategies for the diseases that are associated with changes in the TGFβ signaling.

Keywords: Fibroblasts; Gene expression; Sodium fluoride; Static magnetic field; Transforming growth factor β.