Patch-clamp and multi-electrode array electrophysiological analysis in acute mouse brain slices

Kevin M Manz; Justin K Siemann; Douglas G McMahon; Brad A Grueter

doi:10.1016/j.xpro.2021.100442

Patch-clamp and multi-electrode array electrophysiological analysis in acute mouse brain slices

STAR Protoc. 2021 Apr 5;2(2):100442. doi: 10.1016/j.xpro.2021.100442. eCollection 2021 Jun 18.

Authors

Kevin M Manz^{1

2

3}, Justin K Siemann^{2

4}, Douglas G McMahon^{2

4

5}, Brad A Grueter^{2

3

6

7

8}

Affiliations

¹ Medical Scientist Training Program, Vanderbilt University, Nashville, TN 37232, USA.
² Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN 37232, USA.
³ Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
⁴ Department of Biological Sciences, Vanderbilt University, Nashville, TN 3732, USA.
⁵ Department of Pharmacology, Vanderbilt University, Nashville, TN 37232, USA.
⁶ Vanderbilt Center for Addiction Research, Vanderbilt University, Nashville, TN 37232, USA.
⁷ Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA.
⁸ Anesthesiology Research Division, Vanderbilt University School of Medicine, 2213 Garland Avenue, P435H MRB IV, Nashville, TN 37232-0413, USA.

Abstract

Patch-clamp and multi-electrode array electrophysiology techniques are used to measure dynamic functional properties of neurons. Whole-cell and cell-attached patch-clamp recordings in brain slices can be performed in voltage-clamp and current-clamp configuration to reveal cell-type-specific synaptic and cellular parameters governing neurotransmission. Multi-electrode array electrophysiology can provide spike activity recordings from multiple neurons, enabling larger sample sizes, and long-term recordings. We provide our guide to preparing acute rodent brain slices with example experiments and analyses intended for novice and expert electrophysiologists. For complete details on the use and execution of this protocol, please refer to Manz et al. (2020b).

Keywords: Cell Biology; Neuroscience; Signal Transduction.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Brain* / cytology
Brain* / physiology
Electrophysiology / methods*
Female
Male
Mice
Mice, Inbred C57BL
Neurons
Patch-Clamp Techniques / methods*

Abstract

Publication types

MeSH terms

Grants and funding