miR-139-5p Regulates the Proliferation of Acute Promyelocytic Leukemia Cells by Targeting MNT

Yueyue Fu; Limin Li; Jinxiao Hou; Huibo Li; Chengfang Lv; Hongjuan Yu; Xiaoqian Zhang; Mengyuan Xu; Mingwen Zhang; Hongbin Meng; Jie Liu; Xin Lian; Jiawei Feng; Jin Zhou

doi:10.1155/2021/5522051

miR-139-5p Regulates the Proliferation of Acute Promyelocytic Leukemia Cells by Targeting MNT

J Oncol. 2021 Apr 16:2021:5522051. doi: 10.1155/2021/5522051. eCollection 2021.

Authors

Yueyue Fu¹, Limin Li², Jinxiao Hou¹, Huibo Li¹, Chengfang Lv², Hongjuan Yu², Xiaoqian Zhang², Mengyuan Xu², Mingwen Zhang¹, Hongbin Meng¹, Jie Liu¹, Xin Lian¹, Jiawei Feng¹, Jin Zhou¹

Affiliations

¹ Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, Heilongjiang, China.
² Department of Hematology, South University of Science and Technology Hospital, Shenzhen, China.

Abstract

Acute promyelocytic leukemia (APL) patients with progressive leukocytosis are more likely to have various complications and poor outcomes. However, the regulatory roles of microRNAs in the leukocytosis of APL have not been clarified. Our study aims to evaluate the effects of miRNAs on leukocytosis during induction therapy of APL patients and explore its potential mechanisms. During induction treatment, patients with white blood cell count higher than 10 × 10⁹/L were divided into leukocytosis group and others were nonleukocytosis group. Using microarray assays, we found that miR-139-5p was significantly downregulated in the leukocytosis group. Elevated expression of miR-139-5p inhibited the proliferation of NB4 cells by arresting the cell cycle and inducing apoptosis. We further identified that MNT was a target of miR-139-5p. miR-139-5p significantly inhibited the proliferation, invasion, and migration function of NB4 cells through targeting MNT. Strategies for regulating miR-139-5p or MNT expression might provide new therapeutic approaches for progressive leukocytosis in APL.