Novel Dual-Target μ-Opioid Receptor and Dopamine D3 Receptor Ligands as Potential Nonaddictive Pharmacotherapeutics for Pain Management

J Med Chem. 2021 Jun 10;64(11):7778-7808. doi: 10.1021/acs.jmedchem.1c00611. Epub 2021 May 20.

Abstract

The need for safer pain-management therapies with decreased abuse liability inspired a novel drug design that retains μ-opioid receptor (MOR)-mediated analgesia, while minimizing addictive liability. We recently demonstrated that targeting the dopamine D3 receptor (D3R) with highly selective antagonists/partial agonists can reduce opioid self-administration and reinstatement to drug seeking in rodent models without diminishing antinociceptive effects. The identification of the D3R as a target for the treatment of opioid use disorders prompted the idea of generating a class of ligands presenting bitopic or bivalent structures, allowing the dual-target binding of the MOR and D3R. Structure-activity relationship studies using computationally aided drug design and in vitro binding assays led to the identification of potent dual-target leads (23, 28, and 40), based on different structural templates and scaffolds, with moderate (sub-micromolar) to high (low nanomolar/sub-nanomolar) binding affinities. Bioluminescence resonance energy transfer-based functional studies revealed MOR agonist-D3R antagonist/partial agonist efficacies that suggest potential for maintaining analgesia with reduced opioid-abuse liability.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Analgesics, Opioid / therapeutic use
  • Animals
  • Binding Sites
  • Biphenyl Compounds / chemistry
  • Biphenyl Compounds / metabolism
  • Biphenyl Compounds / therapeutic use
  • Disease Models, Animal
  • Dopamine Antagonists / chemistry*
  • Dopamine Antagonists / metabolism
  • Dopamine Antagonists / therapeutic use
  • Drug Design
  • Fluorescence Resonance Energy Transfer
  • Ligands*
  • Mice
  • Molecular Docking Simulation
  • Opioid-Related Disorders / drug therapy
  • Pain / drug therapy
  • Pain Management
  • Receptors, Dopamine D3 / agonists
  • Receptors, Dopamine D3 / antagonists & inhibitors
  • Receptors, Dopamine D3 / metabolism*
  • Receptors, Opioid, mu / agonists
  • Receptors, Opioid, mu / metabolism*
  • Structure-Activity Relationship

Substances

  • Analgesics, Opioid
  • Biphenyl Compounds
  • Dopamine Antagonists
  • Ligands
  • Receptors, Dopamine D3
  • Receptors, Opioid, mu
  • diphenyl