Chromosome-Level Genome Sequence of Leishmania (Leishmania) tropica Strain CDC216-162, Isolated from an Afghanistan Clinical Case

Yvette Unoarumhi; Dhwani Batra; Mili Sheth; Vidhya Narayanan; Wuling Lin; Yueli Zheng; Lori A Rowe; Jan Pohl; Marcos de Almeida

doi:10.1128/MRA.00842-20

Chromosome-Level Genome Sequence of Leishmania (Leishmania) tropica Strain CDC216-162, Isolated from an Afghanistan Clinical Case

Microbiol Resour Announc. 2021 May 20;10(20):e00842-20. doi: 10.1128/MRA.00842-20.

Authors

Yvette Unoarumhi^{1

2}, Dhwani Batra³, Mili Sheth³, Vidhya Narayanan^{4

5}, Wuling Lin^{4

5}, Yueli Zheng^{4

6}, Lori A Rowe³, Jan Pohl³, Marcos de Almeida⁷

Affiliations

¹ Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, Tennessee, USA.
² Association of Public Health Laboratories (APHL), Silver Spring, Maryland, USA.
³ Biotechnology Core Facility Branch, Division of Scientific Resources, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
⁴ Reference Diagnostic Laboratory, Center for Global Health, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
⁵ IHRC, Inc., Atlanta, Georgia, USA.
⁶ Eagle Global Scientific, San Antonio, Texas, USA.
⁷ Reference Diagnostic Laboratory, Center for Global Health, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia, USA MdeAlmeida@cdc.gov.

Abstract

PacBio and Illumina MiSeq platforms were used for genomic sequencing of a Leishmania (Leishmania) tropica strain isolated from a patient infected in Pakistan. PacBio assemblies were generated using Flye v2.4 and polished with MiSeq data. The results represent a considerable improvement of the currently available genome sequences in the GenBank database.